Part:BBa_K200006

OtsB: Part 2 of 2 coding for trehalose producing enzymes

Sequence codes for trehalose phosphatase enzyme. This enzyme is the second of two required for the conversion of glucose to [http://en.wikipedia.org/wiki/Trehalose trehalose].
This enzyme catalyses the following reaction:

Trehalose is a disaccharide formed from two glucose molecules. Throughout nature, trehalose is associated with resistance to dessication and cold shock otsb1, and is naturally produced in Escherichia Coli.

We hope that by upregulating the trehalose production pathways in E.coli we can increase trehalose concentrations within our cell, thereby conferring some resistance to protein degredation in our system. This would allow easy transport and storage of the final product.

Usage and Biology

Transcription of OtsB gene is activated by osmotic stress in E. coli.otsb1

mRNA of OtsB is more stable at 16°C, therefore, it is a cold inducible mRNA.otsb2

OtsA mutation will block the synthesis of the trehalose-6-phosphase synthase, which is the enzyme that converts glucose-6-phosphate to trehalose-6-phosphate. otsb2

The gene was used alongside OtsA by the Imperial iGEM 2009 [http://2009.igem.org/Team:Imperial_College_London The E.ncapsulator] team as part of the storage protection mechanism. It was also previously used by the UC Berkeley 2007 iGEM team as part of the [http://2007.igem.org/Berkeley_UC Bactoblood] project to enable freeze-drying of the system.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 455
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

<biblio>

1. otsb1 pmid=12105274
2. otsb2 pmid=1310094

</biblio>